Early ovariectomy unmasking the non-somatic origin of murine anti-A reactive IgM release_pbggkataqvbdvi2xnbtt6se6ni

by Peter Arend

Published by Figshare.

2016  

Abstract

The germline encoding of a non-immune IgM molecule in mammals was experimentally documented for the first time by specifically timed ovariectomy of C57BL/10 mice. Although ovariectomy and castrations have been de­scribed to result in uncontrolled and/or enhanced humoral and cellular immunity, involving increasing weights of spleen and thymus with pronounced B and T cell productions, the development of the mercaptoethanol-sensitive and complement-binding, non-immune anti-A reactivity in murine plasma was not enhanced after ovariectomy performed in C57BL/10 mice before the onset of puberty. This non-immune murine anti-A, which is complementary to trans-species, syngeneic ovarian GalNAc-glycan-bearing glycolipids and distinct from cross-reactive adaptive anti-A antibody, was strongly retarded or did not ap­pear at all in plasmas of animals ovariectomized at the age of 20 days. Thus, contrary to our previous view, this reactivity is unlikely to originate from a somatic, primary immune or autoimmune response. All murine tissues expressed the species-typical <i>Forssman</i> reactivity and further A-like structures were, by innate human anti-A antibody, identified in male and female reproductive and endodermal organs, while the murine anti-A was exclusively inhibited by syngeneic ovarian glycolipids. Moreover, the early ovarian tissue, which represents a last evolutionary and/or developmental location, showed a developmental polymorphism characterized by DBL and HPA reactivity indicating the involvement of <i>O</i>-GalNAc-determined mucin-type A-like Tn and TF epitopes that, when expressed by non-developmental tissues, signify malignancy.
In text/plain format

Archived Files and Locations

application/pdf   331.6 kB
file_c6lzjhy74vebxcoxsx42agbnhm
s3-eu-west-1.amazonaws.com (publisher)
web.archive.org (webarchive)
Read Archived PDF
Preserved and Accessible
Type  article-journal
Stage   published
Date   2016-08-08
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 3c6af3c6-d834-4add-8430-6c659a1e6240
API URL: JSON