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Comparison of qNANO results from the isolation of extracellular microvesicles with the theoretical model
release_mzv22bu4tnh6dc7s64xg2jzaiy
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Martyna Durak-Kozica,
Andrzej Wróbel,
Mark Platt,
Ewa Ł. Stępień
Abstract
<jats:title>Abstract</jats:title>
Objectives: Extracellular vesicles (EVs) are heterogeneous membrane vesicles in diameter of 30-5000 nm, that transport proteins, non-coding RNAs (miRNAs), lipids and metabolites. Major populations include exosomes, ectosomes and apoptotic bodies. The purpose of this study was to compare the distribution of EVs obtained under different conditions of differential centrifugation, including ultracentrifugation, with the results developed based on a theoretical model. Methods: Immortalized endothelial cell line that expresses h-TERT (human telomerase) was used to release of EVs: microvascular TIME. EVs were isolated from the culture medium at different centrifugation parameters. The size distribution of the EVs was measured using TRPS technology on a qNano instrument.
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