Effect of crude microcystin on the viable but non-culturable state of Aeromonas sobria in aquatic enviroment release_mkktz5wvvfbtxcpbr6nphgsouq

by PAN Gaoshan, HU Zhangli, LEI Anping, LI Shuangfei

References

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态转变成可培养状态的重要因素之一. Ashton [10] 等在研究有毒甲藻 Ostreopsis lenticularis 与其共生细菌的 关系时发现甲藻藻毒素的存在与细菌 VBNC 状态细菌比率呈负相关关系. 根据本实验结果, 微囊藻毒素
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极有可能对 VBNC 状态细菌起到类似的效果. Dixon [11] 等人以 E.coli(pBR322)为实验材料研究了 MC-LR 对细菌细胞膜通透性的影响. 结果显示, 微囊藻毒素提高了 E.coli 细胞膜对某些物质的通透性. 周立红 [12]
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等人的研究发现, 低浓度藻毒素粗提液对钠钾 ATP 酶有激活作用, 高浓度则有抑制作用. 因此推测细菌 可能在微囊藻毒素的作用下加快吸收微囊藻毒素作为营养物质而进入可培养状态, 又或者激活 ATP 酶而 促使细菌进入更有活力的可培养状态.这些推测只存在理论上的可能性, 还有待进一步验证.
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Xu HS, Singleton F, Conwell et al. Survival and viability of non-culturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. J Microbiol Ecol, 1982, 8: 313-323.
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许 兵, 纪伟尚, 徐怀恕. 一种新发现的细菌的特殊存活形式─-活的非可培养状态. 中国生态学杂志, 1990, 2(3): 144-149.
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Culturability of Stream Bacteria Assessed at the Assemblage and Population Levels
Michael J. Lemke, Laura G. Leff
2006   Microbial Ecology
doi:10.1007/s00248-006-9026-z  pmid:16598638 
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冯 胜, 高 光, 秦伯强等. 太湖北部湖区水体中浮游细菌的动态变化. 湖泊科学, 2006, 18(6): 634-642.
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Porter KG, Feing YS. The use of DAPI for identifying and counting aquatic microflora. J Limnol Oceanogr, 1980, 25(5): 943-948.
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郑桂丽, 廖绍安, 纪伟尚等. 环境中"活的非可培养(VBNC)"细菌的研究进展. 微生物免疫学进展, 2004, 32(4): 58-65.
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Morgan JA, Rhodes G, Pickup RW. Survival of non-culturable Aeromonas salmonicida in lake water. Appl Environ Microbiol , 1993, 59(3): 874-80.
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Thomas TD, Batt RD. Survival of Streptococcus lactisin starvation conditions. J Gen Microbiol , 1968, 50(3): 367-382.
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Ensign JC. Long-term starvation survival of rod and spherical cells of Arthrobacter crystallopoietes. J Bacteriol , 1970, 103 (3): 569-577.
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Ashton M, Rosado W, Tosteson TR et al. Cultural and nonculturable bacterial symbionts in the toxic benthic dinoflagellate ostreopsis lenticularis. J Toxicon, 2003, 42: 419-424.
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Dixon RA, Al-Nazawi M, Alderson G. Permeabilizing effects of sub-inhibitory concentrations of microcystin on the growth of Escherichia coli. FEMS Microbiology Letters, 2004, 230(2): 167-170.
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周立红, 陈学豪. 塔玛亚历山大藻对罗非鱼肝及鳃组织 ATP 酶活性的影响. 海洋科学, 2003, (12): 75-78.