@misc{shen_hartmann_2022, 
  title={DNA extraction protocol for low-biomass environmental samples: adapted from the Lucigen MasterPure Complete DNA and RNA Purification Kit manual}, 
  DOI={10.21203/rs.3.pex-1658/v1}, 
  abstractNote={<jats:title>Abstract</jats:title>
        <jats:p>Microbial samples collected from built environment surfaces can be very low-biomass. This poses a critical challenge to extract valid signals out of the background noises for downstream analyses. Upon comparing and optimizing several extraction methods, our results demonstrated that bead-beating and heat lysis followed by liquid-liquid extraction was the optimal method, as opposed to widely used column- and magnetic bead-based methods. Adapted from the Lucigen MasterPure Complete DNA and RNA Purification Kit manual, this protocol demonstrates detailed points that merit special attention for low-biomass environmental samples.</jats:p>}, 
  publisher={Research Square Platform LLC}, 
  author={Shen, Jiaxian and Hartmann, Erica M.}, 
  year={2022}, 
  month={Feb}
  }