Development of surface‐enhanced Raman scattering ‐based immunoassay platforms using hollow Au nanostars for reliable SARS‐CoV ‐2 diagnosis release_iy77qm3wefffzcjiqqvzaz2m5i

by Qian Yu, Yixuan Wu, Taejoon Kang, Jaebum Choo

Published in Bulletin of the Korean Chemical Society (Print) by Wiley.

2021  

Abstract

We developed a novel surface-enhanced Raman scattering (SERS)-based SARS-CoV-2 assay platform using hollow Au nanostars to realize high-sensitivity diagnosis of SARS-CoV-2. The assay was performed using SARS-CoV-2 lysate as the target in a wide dynamic range with virus concentrations ranging from 0 to 104 PFU/ml and has a limit of detection (LOD) of 5.1 PFU/ml. This LOD value shows 100 times and 10 times better sensitivity compared to the LODs measured on the same sample using a commercially available rapid kit and enzyme-linked immunosorbent assay, respectively. Therefore, we believe that this SERS-based SARS-CoV-2 assay platform has high diagnostic accuracy for early or asymptomatic infected patients with low virus concentrations. Furthermore, the probability of a false-negative diagnosis is likely to be very low.
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Type  article-journal
Stage   published
Date   2021-10-14
Language   en ?
DOI  10.1002/bkcs.12418
PubMed  34898787
PMC  PMC8653018
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