BibTeX
CSL-JSON
MLA
Harvard
Isolation of Human Dental Pulp Stem Cells and Their Characteristics Before and After Cryopreservation
release_hhcd6irnerdhpnilhbbpw2ee2q
by
Svitlana Mazur,
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv,
Olena Rogulska,
Olena Revenko,
Nataliya Volkova,
Alexander Petrenko,
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv,
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine,
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine,
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
Published
in Problems of Cryobiology and Cryomedicine
by National Academy of Sciences of Ukraine (Co. LTD Ukrinformnauka).
2021 Volume 31, p58-69
Abstract
Dental pulp stem cells (DPSCs) from human third molar tooth germ (wisdom tooth) were isolated using a collagenasebased enzymatic method, the obtained cells were analyzed as for morphology in monolayer culture, immunophenotype, proliferation and differentiation potential before and after cryopreservation. In this study, we showed that based on morphological features, surface markers profile and differentiation potential, the isolated DPSCs corresponded to multipotent mesenchymal stromal cells. DPSCs cryopreservation by slow cooling (1 °С / min) down to –80°C with subsequent immersion into liquid nitrogen in cryoprotectant-free culture medium led to cell death. Cryopreservation using the same protocol in the presence of 10% dimethyl sulfoxide (DMSO) and 20% serum ensured (82 ± 6)% cell viability; while metabolic and proliferative activity, as well as the ability to differentiate into the osteo- and adipogenic lineages of cryopreserved DPSCs were similar to their non-cryopreserved counterparts.
In application/xml+jats format
Archived Files and Locations
application/pdf
921.2 kB
file_bqbhzduwnjesvi7zznzuajxpqi
|
cryo.org.ua (web) web.archive.org (webarchive) |
Read Archived PDF
Preserved and Accessible
Type
Stage
Date 2021-03-30
article-journalStage
published
Date 2021-03-30
Container Metadata
Open Access Publication
Not in DOAJ
In ISSN ROAD
Not in Keepers Registry
ISSN-L:
Open Access Publication
Not in DOAJ
In ISSN ROAD
Not in Keepers Registry
ISSN-L:
2307-6143
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
access all versions, variants, and formats of this works (eg, pre-prints)
Cite This
Lookup Links
oaDOI/unpaywall (OA fulltext)
Crossref Metadata (via API)
Worldcat
SHERPA/RoMEO (journal policies)
wikidata.org
CORE.ac.uk
Semantic Scholar
Google Scholar
Crossref Metadata (via API)
Worldcat
SHERPA/RoMEO (journal policies)
wikidata.org
CORE.ac.uk
Semantic Scholar
Google Scholar