MiR 494 inhibits the proliferation, migration and invasion of cervical cancer cells by regulating LETMD1 release_gxpjd2ntj5eitfbp4z3ejxci4e

Abstract

This study focused on miR-494 inhibiting the proliferation, migration and invasion of cervical cancer cells by regulating LETMD1. During the study, 30 pairs of primary cervical cancer tissues and adjacent tissues diagnosed by pathology were collected, and then placed in a cryopreservation tube, immediately placed in liquid nitrogen to freeze, and then moved to a -80 °C refrigerator for storage until use. Dip with crystal violet staining solution for 20 minutes, wash 3 times with PBS, and dry at room temperature. The polycarbonate film on the chamber was gently cut, placed on a glass slide, and covered with neutral resin. The number of cells in 5 random fields was counted under a microscope, and the differences between the groups were compared. The qRT-PCR results showed that compared with the normal cervical tissue cell line LETMD1, the expression levels of miR-494 in cervical cancer cell lines LETMD1 and C-33A were significantly reduced. Compared with the transfection of mimicsNC, the level of LETMD1mRNA and protein decreased significantly when transfected with miRNA-494mimics (P <0.05); compared with the transfection of inhibitorNC, the level of LETMD1mRNA and protein increased significantly when transfected with miRNA-494 inhibitor (P <0.05).
In application/xml+jats format

Archived Files and Locations