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Identification of Single Nucleotide Polymorphisms in Selenium-Glutathione Peroxidase (GPX1) Gene
release_equk5fcolzavphzuib6jaschfa
by
Ala A. Qatatsheh,
Rula Amr,
Murad A. Al-Holy,
Amin N. Olaimat,
R. Ibrahim
Published
in Current Research in Nutrition and Food Science Journal
by Enviro Research Publishers.
2019 p819-827
Abstract
Human genetic variation is quite common with single nucleotide polymorphisms (SNPs) accounting for the majority of the variants. In the present study, primers for amplification of the appropriate part of the human GPX1 gene by polymerase chain reaction (PCR) were designed. The aim of this study was to develop a restriction fragment length polymorphism (RFLP) assay to detect and characterize GPX1 polymorphism in the coding region and validate the assays by sequencing.
This study demonstrated that the RFLP method, which can be rapid, is reliable and valid as a tool for identifying the different polymorphisms with a high degree of specificity and sensitivity.
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2322-0007
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