Autophagy regulation and anti-inflammatory effect of curcumin on the mouse with experimental autoimmune encephalomyelitis release_ddhd6xzpabavfj37mfksxqcgau

Abstract

Objective To investigate the anti-inflammatory effect of curcumin on the mouse with experimental autoimmune encephalomyelitis (EAE), and explore its mechanism of action. Methods Forty female C57BL/6 mice were randomly divided into EAE+DMSO group, EAE+curcumin group, DMSO group and curcumin group with10 mice in each group. Mice in EAE+DMSO group and EAE+curcumin group were used to establish the EAE model. DMSO group was the solvent control of EAE+DMSO group, and 0.2 ml 20% DMSO solution daily were intraperitoneally injected in the both groups a week after immunization; and mice in curcumin group and EAE+curcumin group were daily intraperitoneally injected with 10 mg/kg curcumin solution a week after immunization. From the date of immunization, the mice in each group were evaluated for clinical symptoms every day. Mice were anesthetized and sacrificed at the peak period of EAE (21 d), their spinal cord tissues were taken for HE staining, serum concentrations of INF-γ and IL-17 were measured by ELISA, spleen lymphocytes were sorted for CD4+ T. The expression levels of Atg-5 mRNA and LC3-Ⅱ mRNA in spinal cord tissue were determined by RT-PCR, and of LC3-Ⅱ was estimated by immunohistochemical staining. Results Based on the successful establishment of mouse EAE model, there were no obvious neurological injury symptoms in DMSO group and curcumin group, and no inflammatory reaction by HE staining in spinal cord tissue. The clinical symptom score in EAE+DMSO group (3.312±0.347) was significantly higher than that in DMSO group (P<0.05), and HE staining of spinal cord tissue indicated massive inflammatory cell infiltration and local vascular cuff appearance. Compared with the EAE+DMSO group, the clinical symptom score of EAE+curcumin group (1.562±0.463) was significantly lower (P<0.05), and the infiltration of inflammatory cells in HE stained spinal cord tissue was reduced significantly without typical vascular cuff like changes. For mice in DMSO group and curcumin group, the percentages of CD4+ T cells (39.76%±6.51%, [...]
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