@misc{iranpour_shahsavarani_hosseini_far_zhand_ghanbarlou_bouzari_shokrgozar_2021, title={Self-assembling of Chimeric Mussel-inspired Bio-adhesives Originated from Mytilus Californianus and Anabaena Flos-aquae: A New Approach to Develop Underwater Adhesion}, DOI={10.21203/rs.3.rs-294097/v1}, abstractNote={Abstract Bio-adhesives play a pivotal role in a wide range of medical applications. However, there are some problems about their application in varied pH values and low adhesion force under wet conditions. Here, we report new recombinant fusion protein achieved by mussel foot proteins (Mfps) of Mytilus Californianus and gas vesicle protein A (GvpA) of Anabaena flos-aquae by genetic engineering methods. These chimeric proteins self-assembled into ß-sheet rich fibres because of GvpA amyloid structure. Also, their adhesion forces were significantly increased especially in alkaline environment based on Mfp-3 and Mfp-5. This study illustrates that copolymer of Mfp-5-GvpA:GvpA-Mfp-3 can be used as an underwater sturdy adhesive with tolerance to auto-oxidation, especially at basic conditions.}, publisher={Research Square}, author={Iranpour, Hamidreza and Shahsavarani, Hossein and Hosseini, Seyed Nezamedin and Far, Hani Hosseini and Zhand, Sareh and Ghanbarlou, Mahdi Mohammadi and Bouzari, Saeid and Shokrgozar, Mohammad Ali}, year={2021}, month={Mar} }