@article{arakawa_yphantis_1987, 
  title={Molecular weight of recombinant human tumor necrosis factor-alpha}, 
  volume={262}, 
  abstractNote={Recombinant DNA-derived human tumor necrosis factor-alpha from Escherichia coli was examined by equilibrium ultracentrifugation under conditions similar to those where gel filtration experiments suggested an oligomeric structure. Short-column equilibrium experiments at concentrations in the range 0.015-0.12% at pH 8.5 in 0.04 M Tris/Tris-HCl gave molecular weights corresponding to 3 times the sequence molecular weight both in the presence and absence of 0.1 M NaCl. Long (2.6 mm)-column experiments under the same solvent conditions indicated molecular weights of 51,900 +/- 900 in the absence of added NaCl and 52,600 +/- 700 in the presence of added 0.1 M NaCl. No evidence of any species other than the trimer was found.}, 
  number={16}, 
  author={Arakawa, T and Yphantis, D A}, 
  year={1987}, 
  month={Jun}
  }