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Production of Recombinant Monoclonal Antibodies in the Egg White of Gene-Targeted Transgenic Chickens release_5rc37vz5draovkcakaxj4hlymu

by Takehiro Mukae, sho okumura, Takuma Watanobe, Kyoko Yoshii, Takahiro Tagami, Isao Oishi

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abstracts[] {'sha1': 'bfa31066c125561ef368ff2d27c3163519d89a2b', 'content': 'Increased commercial demand for monoclonal antibodies (mAbs) has resulted in the urgent need to establish efficient production systems. We previously developed a transgenic chicken bioreactor system that effectively produced human cytokines in egg whites using genome-edited transgenic chickens. Here, we describe the application of this system to mAb production. The genes encoding the heavy and light chains of humanized anti-HER2 mAb, linked by a 2A peptide sequence, were integrated into the chicken ovalbumin gene locus using a CRISPR/Cas9 protocol. The knock-in hens produced a fully assembled humanized mAb in their eggs. The mAb expression level in the egg white was 1.4–1.9 mg/mL, as determined by ELISA. Furthermore, the antigen binding affinity of the anti-HER2 mAb obtained was estimated to be equal to that of the therapeutic anti-HER2 mAb (trastuzumab). In addition, antigen-specific binding by the egg white mAb was demonstrated by immunofluorescence against HER2-positive and -negative cells. These results indicate that the chicken bioreactor system can efficiently produce mAbs with antigen binding capacity and can serve as an alternative production system for commercial mAbs.', 'mimetype': 'application/xml+jats', 'lang': None}
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issue 1
language en
license_slug CC-BY
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pages 38
publisher MDPI AG
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release_date 2020-12-30
release_stage published
release_type article-journal
release_year 2020
subtitle
title Production of Recombinant Monoclonal Antibodies in the Egg White of Gene-Targeted Transgenic Chickens
version
volume 12
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As JSON via API

Extra Metadata (raw JSON)

crossref.alternative-id ['genes12010038']
crossref.funder [{'DOI': '10.13039/501100001691', 'award': ['16K08093', '20K21377', '20H03135'], 'doi-asserted-by': 'publisher', 'name': 'Japan Society for the Promotion of Science'}]
crossref.license [{'URL': 'https://creativecommons.org/licenses/by/4.0/', 'content-version': 'vor', 'delay-in-days': 0, 'start': '2020-12-30T00:00:00Z'}]
crossref.subject ['Genetics(clinical)', 'Genetics']
crossref.type journal-article
pubmed.pub_types ['Journal Article']