Reference genome-assisted identification of stem rust resistance gene Sr62 encoding a tandem kinase

Guotai Yu; Oadi Matny; Nicolas Champouret; Burkhard Steuernagel; Matthew J. Moscou; Inmaculada Hernández-Pinzón; Phon Green; Sadiye Hayta; Mark Smedley; Wendy Harwood; Ngonidzashe Kangara; Yajuan Yue; Catherine Gardener; Mark J. Banfield; Pablo D. Olivera; Cole Welchin; Jamie Simmons; Eitan Millet; Anna Minz-Dub; Moshe Ronen; Raz Avni; Amir Sharon; Mehran Patpour; Annemarie F. Justesen; Murukarthick Jayakodi; Axel Himmelbach; Nils Stein; Shuangye Wu; Jesse Poland; Jennifer Ens; Curtis Pozniak; Miroslava Karafiátová; István Molnár; Jaroslav Doležel; Eric R. Ward; T Lynne Reuber; Jonathan D. G. Jones; Martin Mascher; Brian J. Steffenson; Brande B. H. Wulff

doi:10.21203/rs.3.rs-1198968/v1

by Guotai Yu, Oadi Matny, Nicolas Champouret, Burkhard Steuernagel, Matthew J. Moscou, Inmaculada Hernández-Pinzón, Phon Green, Sadiye Hayta, Mark Smedley, Wendy Harwood, Ngonidzashe Kangara, Yajuan Yue (+28 others)

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2021

Abstract

<jats:title>Abstract</jats:title> The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (<jats:italic>R</jats:italic>) genes. Molecular identification and characterization of these <jats:italic>R</jats:italic> genes facilitates their manipulation and tracking in breeding programmes. We developed a reference-quality genome assembly of the wild diploid wheat relative <jats:italic>Aegilops sharonensis</jats:italic> and used positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene <jats:italic>Sr62</jats:italic>, which was also transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable <jats:italic>Sr62</jats:italic> transgenic wheat lines showed high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of <jats:italic>Sr62</jats:italic> for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.
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Date 2021-12-29

DOI 10.21203/rs.3.rs-1198968/v1

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Reference genome-assisted identification of stem rust resistance gene Sr62 encoding a tandem kinase release_2svz2urigzgojdkhqt5fz66rhm

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